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pgadt7 vector  (TaKaRa)


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    Structured Review

    TaKaRa pgadt7 vector
    Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgadt7+ad+vectors/pGADT7+AD+Vector/pmc13188844-81-52-54
    Average 99 stars, based on 3975 article reviews
    pgadt7 vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae
    Article Snippet: .. Fragments were cloned into 206 pGBKT7 (BD) and pGADT7 (AD) vectors, respectively, by in-fusion cloning (In-Fusion Cloning kit; 207 Clontech Laboratories). pGBKT7(BD)-N-Rgs1, pGBKT7(BD)-Rgs1 were used to test 208 transactivation activity, and pGADT7(AD)- N-Rgs1, pGADT7(AD)-Rgs1 were used to test DNA-209 binding ability. ..

    Article Title: Receptor-like kinases OsRLK902-1 and OsRLK902-2 form immune complexes with OsRLCK185 to regulate rice blast resistance.
    Article Snippet: Receptor-like kinases (RLKs) are major regulators of the plant immune response and play important roles in the perception and transmission of immune signals.. RECEPTOR LIKE KINASE 902 (RLK902) is at the key node in leucinerich repeat receptor-like kinase interaction networks and positively regulates resistance to the bacterial pathogen Pseudomonas syringae in Arabidopsis.. However, the function of RLK902 in fungal disease resistance remains obscure.

    Article Title: Control of meiotic crossover interference by a proteolytic chaperone network.
    Article Snippet: Meiosis is a specialized eukaryotic division that produces genetically diverse gametes for sexual reproduction.. During meiosis, homologous chromosomes pair and undergo reciprocal exchanges, called crossovers, which recombine genetic variation.. Meiotic crossovers are stringently controlled with at least one obligate exchange forming per chromosome pair, while closely spaced crossovers are inhibited by interference.

    Article Title: Site-specific phosphorylation of LRP regulates FLC chromatin looping and flowering
    Article Snippet: .. The coding sequences of various genes were amplified and cloned into pGBKT7 (BD) or pGADT7 (AD) vectors (Clontech) using the ClonExpress II One Step Cloning Kit (Vazyme). .. Various combinations of AD and BD vectors were cotransformed into AH109 cells using the Yeastmaker Yeast Transformation System 2 (Clontech).

    Cloning:

    Article Title: Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae
    Article Snippet: .. Fragments were cloned into 206 pGBKT7 (BD) and pGADT7 (AD) vectors, respectively, by in-fusion cloning (In-Fusion Cloning kit; 207 Clontech Laboratories). pGBKT7(BD)-N-Rgs1, pGBKT7(BD)-Rgs1 were used to test 208 transactivation activity, and pGADT7(AD)- N-Rgs1, pGADT7(AD)-Rgs1 were used to test DNA-209 binding ability. ..

    Article Title: Control of meiotic crossover interference by a proteolytic chaperone network.
    Article Snippet: Meiosis is a specialized eukaryotic division that produces genetically diverse gametes for sexual reproduction.. During meiosis, homologous chromosomes pair and undergo reciprocal exchanges, called crossovers, which recombine genetic variation.. Meiotic crossovers are stringently controlled with at least one obligate exchange forming per chromosome pair, while closely spaced crossovers are inhibited by interference.

    Article Title: Site-specific phosphorylation of LRP regulates FLC chromatin looping and flowering
    Article Snippet: .. The coding sequences of various genes were amplified and cloned into pGBKT7 (BD) or pGADT7 (AD) vectors (Clontech) using the ClonExpress II One Step Cloning Kit (Vazyme). .. Various combinations of AD and BD vectors were cotransformed into AH109 cells using the Yeastmaker Yeast Transformation System 2 (Clontech).

    Activity Assay:

    Article Title: Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae
    Article Snippet: .. Fragments were cloned into 206 pGBKT7 (BD) and pGADT7 (AD) vectors, respectively, by in-fusion cloning (In-Fusion Cloning kit; 207 Clontech Laboratories). pGBKT7(BD)-N-Rgs1, pGBKT7(BD)-Rgs1 were used to test 208 transactivation activity, and pGADT7(AD)- N-Rgs1, pGADT7(AD)-Rgs1 were used to test DNA-209 binding ability. ..

    Binding Assay:

    Article Title: Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae
    Article Snippet: .. Fragments were cloned into 206 pGBKT7 (BD) and pGADT7 (AD) vectors, respectively, by in-fusion cloning (In-Fusion Cloning kit; 207 Clontech Laboratories). pGBKT7(BD)-N-Rgs1, pGBKT7(BD)-Rgs1 were used to test 208 transactivation activity, and pGADT7(AD)- N-Rgs1, pGADT7(AD)-Rgs1 were used to test DNA-209 binding ability. ..

    Article Title: Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities
    Article Snippet: .. To confirm the interactions and narrow down the binding sites, pGBKT DNA-BD and pGADT7-AD vectors (Takara Bio) containing cDNAs encoding regions of MYPN, PALLD, CARP, and FHOD1 were cotransformed into the yeast two-hybrid Gold yeast strain and spotted on SD/–Ade/–His/–Leu/–Trp/AbA/X-α-Gal plates. ..

    Article Title: Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities
    Article Snippet: .. To confirm the interactions and narrow down the binding sites, pGBKT DNA- BD and pGADT7- AD vectors (Takara Bio) containing cDNAs encoding regions of MYPN, PALLD, CARP, and FHOD1 were cotransformed into the yeast two- hybrid Gold yeast strain and spotted on SD/–Ade/–His/–Leu/–Trp/AbA/X-α-Gal plates. ..

    Plasmid Preparation:

    Article Title: DRN facilitates WUS transcriptional regulatory activity by chromatin remodeling to regulate shoot stem cell homeostasis in Arabidopsis
    Article Snippet: After being washed 3 times with pull-down buffer, the resin beads were boiled and processed for western blotting analyses using anti-GST (catalog no. M20025, Abmart) and anti-His (catalog no. M20020, Abmart) antibodies. .. The pGBKT7 (BD) and pGADT7 (AD) vectors (Clontech) were used for plasmid construction. .. The combinations of AD and BD were transformed into the yeast strain Y2H Gold, which was cultured on complete medium lacking leucine and tryptophan (SD/−Leu/−Trp).

    Sterility:

    Article Title: Receptor-like kinases OsRLK902-1 and OsRLK902-2 form immune complexes with OsRLCK185 to regulate rice blast resistance.
    Article Snippet: Receptor-like kinases (RLKs) are major regulators of the plant immune response and play important roles in the perception and transmission of immune signals.. RECEPTOR LIKE KINASE 902 (RLK902) is at the key node in leucinerich repeat receptor-like kinase interaction networks and positively regulates resistance to the bacterial pathogen Pseudomonas syringae in Arabidopsis.. However, the function of RLK902 in fungal disease resistance remains obscure.

    Suspension:

    Article Title: Receptor-like kinases OsRLK902-1 and OsRLK902-2 form immune complexes with OsRLCK185 to regulate rice blast resistance.
    Article Snippet: Receptor-like kinases (RLKs) are major regulators of the plant immune response and play important roles in the perception and transmission of immune signals.. RECEPTOR LIKE KINASE 902 (RLK902) is at the key node in leucinerich repeat receptor-like kinase interaction networks and positively regulates resistance to the bacterial pathogen Pseudomonas syringae in Arabidopsis.. However, the function of RLK902 in fungal disease resistance remains obscure.

    Selection:

    Article Title: Receptor-like kinases OsRLK902-1 and OsRLK902-2 form immune complexes with OsRLCK185 to regulate rice blast resistance.
    Article Snippet: Receptor-like kinases (RLKs) are major regulators of the plant immune response and play important roles in the perception and transmission of immune signals.. RECEPTOR LIKE KINASE 902 (RLK902) is at the key node in leucinerich repeat receptor-like kinase interaction networks and positively regulates resistance to the bacterial pathogen Pseudomonas syringae in Arabidopsis.. However, the function of RLK902 in fungal disease resistance remains obscure.

    Amplification:

    Article Title: Site-specific phosphorylation of LRP regulates FLC chromatin looping and flowering
    Article Snippet: .. The coding sequences of various genes were amplified and cloned into pGBKT7 (BD) or pGADT7 (AD) vectors (Clontech) using the ClonExpress II One Step Cloning Kit (Vazyme). .. Various combinations of AD and BD vectors were cotransformed into AH109 cells using the Yeastmaker Yeast Transformation System 2 (Clontech).



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